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HCV genotyping Hepatitis C is a heterogeneous virus with at least 6 genotypes and numerous subtypes identified around the world.47,48 Although considerable disagreement exists on the natural history of the disease in patients infected with different genotypes, there is a general consensus on the fact that the HCV genotype is one of the most important predictors to antiviral therapy response.14,42,49 The gold standard for genotyping is the direct sequencing of the NS5B or E1 region, followed by the sequence alignment with reference sequences and phylogenetic analysis.50 In clinical practice, HCV can be geno-typed with several methods such as: 1) restriction fragment length polymorphism (RFLP) analysis of the highly conserved 5 non coding region;51 2) nested PCR analysis of the HCV core region using genotype-specific primers;52 3) reverse hybridization analysis using genotype-specific probes of 5 non coding region sequences;53 and 4) direct sequencing of 5 non coding region and sequence comparison with reference database.54 The first method is rapid and inexpensive, but lacks standardization while the second is labour-intensive and yields to high rates of cross amplification
[DOI] [PMC free article] [PubMed] [Google Scholar] 54.Griffith O.W., Anderson M.E., Meister A
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