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The mustard seed extract was testified for APAP-induced toxicity in a hepatocellular carcinoma cell line (HepG2) and was found to have protective effects in both post and pretreatment models with a 20 mM APAP dose (Parikh et al., 2015)

Azoxymethane treatment of Sec-tRNA [Ser] Sec i6A transgenic mice with reduced expression of GPx1 resulted in aberrant crypts in their colon compared to wild-type mice

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The assay measures the increase in absorbance monitored at 340 nm associated with the formation of NADPH, which is proportional to the aconitase activity