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Since carnitine is a polar compound, the addition of organic solvents during the analyte separation process will increase the solubility of carnitines in the mobile phase and alter the characteristics of the stationary phase through adsorption, thereby changing retention, selectivity, and efficiency

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doi: 10.1016/j.anaerobe.2019.102138 132 WuW.LvL.ShiD.YeJ.FangD.GuoF.et al

It also contains sulphites and tannins, both of which can trigger additional reactions