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Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles

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Beyond intestinal dysbiosis, recent research has shown that microbial metabolites influence immunological tone by acting on receptors such as GPR41/43, AhR, and FXR, regulating cytokine secretion, T-cell differentiation, and microglial activity [100]