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Research has consistently shown that, in individuals with normal renal function, long-term creatine use does not lead to any significant changes in key kidney markers, such as glomerular filtration rate (GFR) or serum creatinine levels, beyond the temporary increase seen during supplementation [80]

Ethics statement The studies involving humans were approved by Ethics Committee of Beijing Anzhen Hospital of the Capital University of Medical Sciences

Temperature fluctuations particularly promote aggregation as peptides unfold and refold repeatedly

It has also been shown in numerous species, including primates, that caloric restriction prolongs the lifespan (127, 128)