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Mice undergoing in vivo reprogramming were found to become depleted in B 12 and show signs of methionine starvation while supplementing reprogramming mice and cells with B 12 increased reprogramming efficiency, indicating a cell-intrinsic effect

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10.1073/pnas.2435457100 145

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The new PepD construct (BBa_K3206015) was designed to have lower expression using a Bba_I1051 promoter

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