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Osmolarity was restored by adding 20 mL plain RPMI, followed by centrifugation at 10,000 rpm for 5 min

Neurochem Int 76: 5969

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Determination of adenosine triphosphate in kidney tissue Adenosine triphosphate in renal samples was determined based on the method previously described by Botker et al., [40] utilizing an HPLC system (Jasco Corporation, Ishikawa-Cho, Hachioji, Tokyo, Japan)